Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin-carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces strongly dominate over the non-specific ones. However, obtaining quantitative results requires the complex and tedious calibration of a torsional...

Probing Biomolecular Interactions Using Nanopore Force Spectroscopy

Probing Nanoscale Heat and Force Interactions Using Atomic Force Microscopes (afm)

Many devices and instruments such as magnetic hard disk drives and atomic force microscopes (AFM) rely on the stable operation of their small probing heads at nanoscale gaps. Due to the small scale of the probing heads, the force interactions (Casimir force and electrostatic force) between the small probes and the surrounding become more significant. The local heating caused by read/write elect...

Single Molecule Probing of Exocytotic Protein Interactions Using Force Spectroscopy.

Relatively recently, the Atomic Force Microscope (AFM) emerged as a powerful tool for single molecule nanomechanical investigations. Parameters that can be measured by force spectroscopy using AFM, such as the force and total mechanical extension required to break bonds between various proteins can yield valuable insights into the nature of the bond (zippering vs. highly localized binding site)...

Probing Interactions within the synaptic DNA-SfiI complex by AFM force spectroscopy.

SfiI belongs to a family of restriction enzymes that function as tetramers, binding two recognition regions for the DNA cleavage reaction. The SfiI protein is an attractive and convenient model for studying synaptic complexes between DNA and proteins capable of site-specific binding. The enzymatic action of SfiI has been very well characterized. However, the properties of the complex before the...